Cell exposure
Laboratory experiments measured markers after controlled in-vitro exposure.

Protocol / Research Dosing Guide
An evidence-organized Cartalax reference separating AED cell-culture research from unvalidated community dosing and product claims.
Cartalax is the tripeptide Ala-Glu-Asp, abbreviated AED. Research has examined cell-culture markers related to cartilage and aging, but no controlled human trial, human pharmacokinetic study, or validated clinical regimen was identified.
Why researchers care
Cartilage, connective-tissue, and cell-aging research
Class
Synthetic AED tripeptide bioregulator
Route reported
Subcutaneous use is community reported
Cycle length
Up to 12 weeks reported; not validated
Regulatory status
Not FDA approved
The supplied source presents a 2-to-5 mg daily, 12-week community titration and also mentions a separate 10 mg for 20 days pattern. Neither schedule comes from a controlled human dose-finding study.
| Phase or study | Amount | Frequency / evidence |
|---|---|---|
| Weeks 1–2 | 2 mg daily | Community reported |
| Weeks 3–4 | 3 mg daily | Community reported |
| Weeks 5–8 | 4 mg daily | Community reported |
| Weeks 9–12 | 5 mg daily | Community reported |
The 200 ng/mL concentration used in cell experiments cannot be converted into a human dose. No human amount, cycle, or rest period is established.
Neutral research-material planning only. This does not establish an appropriate amount or route.
Research planning item
Exact labeled research vial
Research planning item
Measured diluent volume
Research planning item
U-100 measurement reference
Research planning item
Alcohol swabs
Research planning item
Appropriate sharps container
Research planning item
Written concentration label
Concentration
6.667 mg/mL
Draw volume
0.3 mL
U-100 units
30
Mathematical draws
10
Arithmetic only. The entered amount is not a recommendation and does not establish suitability.
Not established; source timing is community convention.
Daily use is community reported and not trial validated.
Not applicable to the reported vial route and not established.
No validated replacement or doubling instructions exist.
| Route | Research context | Evidence limit |
|---|---|---|
| Cell culture | Human stem cells and fibroblasts | In vitro, not clinical exposure |
| Isolated rat chondrocytes | Preclinical tissue research | Not whole-animal dosing |
| Subcutaneous | Community reported | No human route study |
| Oral or lingual | Commercial products reported | Formulations and exposure not established |
Routes and amounts are not interchangeable unless a verified study explicitly establishes a conversion.
| Measure | Finding | Evidence limit |
|---|---|---|
| Human half-life | Not established | No human PK study |
| Animal half-life | Not established | No in-vivo PK study located |
| Bioavailability | Not established | No route-specific exposure data |
| Frequency | Daily is community reported | Not experimentally validated |
| Route | Amount | Frequency | Source | Human validation |
|---|---|---|---|---|
| Subcutaneous | 2 mg | Daily, weeks 1–2 | Community report | No |
| Subcutaneous | 3 mg | Daily, weeks 3–4 | Community report | No |
| Subcutaneous | 4 mg | Daily, weeks 5–8 | Community report | No |
| Subcutaneous | 5 mg | Daily, weeks 9–12 | Community report | No |
| Cell culture | 200 ng/mL | Experimental concentration | In vitro research | Not convertible |
Published study values describe those studies. Community-reported values are not validated dosing recommendations.
Concentration: 6.667 mg/mL
| Entered amount | Volume | U-100 units |
|---|---|---|
| 2 mg | 0.3 mL | 30 units |
| 4 mg | 0.6 mL | 60 units |
| 6 mg | 0.9 mL | 90 units |
Reconstitution does not establish identity, purity, sterility, stability, or an appropriate amount.
Cartalax is the short peptide Ala-Glu-Asp.
Current status · verified September 15, 2026
Not FDA approved
This is an evidence-gap and precaution list, not an approved prescribing label.
Pregnancy or breastfeeding
No safety data
Children or adolescents
No safety data
Joint disease needing diagnosis
No demonstrated clinical efficacy
Known ingredient sensitivity
Potential allergic risk
Concurrent medicines
No interaction studies
Long-term or repeated use
No safety data
Important limitation: No controlled incidence data.
Important limitation: No human safety dataset.
Important limitation: No characterized immunogenicity data.
Important limitation: No interaction studies.
Important limitation: No pregnancy or fertility data.
Important limitation: No follow-up evidence.
Laboratory experiments measured markers after controlled in-vitro exposure.
Community titration period, not a clinical study duration.
A separate community schedule; not validated.
Not established; no restart criteria or cumulative-exposure limits exist.
| Claim | Evidence level | What research shows | Important limitation |
|---|---|---|---|
| Cartilage differentiation markers | Laboratory evidence | Human stem-cell models | Does not establish joint outcomes |
| Chondrocyte activity | Preclinical | Isolated rat-cell experiments | Not whole-animal efficacy |
| Skin-fibroblast aging markers | Laboratory evidence | In-vitro gene-expression studies | Clinical meaning unknown |
| Joint pain or mobility | Not established | Community claim | No controlled human trial |
No Cartalax-specific stability study was identified.
Community refrigeration guidance is not validated stability evidence.
Formulations may contain other ingredients and are not interchangeable.
Visual inspection cannot establish identity, purity, or sterility.
Stop and seek qualified product guidance; appearance cannot confirm sterility.
Recheck vial mg, liquid mL, target mg, and U-100 conversion.
Do not convert 200 ng/mL in vitro into a human amount.
The formulations and routes are not interchangeable.
No validated missed-amount procedure exists.
| Compound | Class or mechanism | Evidence | FDA status |
|---|---|---|---|
| Cartalax | AED tripeptide | Cell-culture/preclinical evidence | Not approved |
| Cardiogen | AEDR tetrapeptide | Limited preclinical evidence | Not approved |
| BPC-157 | Synthetic pentadecapeptide | Primarily animal evidence | Not approved |
The synthetic tripeptide Ala-Glu-Asp, also called AED.
No.
No controlled human Cartalax trial was identified.
Not established.
No; it is community reported.
No.
No.
No.
No.
It performs concentration and volume arithmetic only.
Coverage source
Coverage source for community schedules; not primary clinical evidence.
Open direct sourceLaboratory research
Human stem-cell laboratory research using AED.
Open direct sourceLaboratory research
In-vitro human fibroblast study; not a clinical trial.
Open direct sourcePreclinical review
Review of short-peptide preclinical research.
Open direct source